Anti-Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)

Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican contains an N-terminal V-type Ig-like domain (aa52-139), a C-terminal EGF-like (aa646-682) and C-type lectin domain (aa695-802). Multiple isoforms exist where there is an alternate start site at Met228 and a 23aa substitution for aa 649-911 that serves as a GPI-anchor motif. over aa 23-911, human Brevican shares 82% aa identity with mouse Brevican. Applications: Suitable for use in ELISA and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 10ug/ml detects Brevican on rat cortical neural stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted B2739-70C overnight at 4°C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA., Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Brevican (aa23-911). Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.