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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
B2739-70B.100 | 100 µg | - | - |
3 - 19 business days* |
901.00€
|
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You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican... more
Product information "Anti-Brevican (BEHAB, Brain-Enriched Hyaluronan-Binding Protein)"
Brain-enriched hyaluronan-binding protein (Brevican) is a 160kD member of the aggregan/versican proteoglycan family of matrix proteins. It is brain-derived and serves as a linker between HA and other matrix molecules such as tenascins and fibulins. Mature human Brevican is 889 amino acids in length. It contains multiple domains, with an N-terminal V-type Ig-like domain (aa52-139) and a C-terminal EGF-like (aa646-682) and C-type lectin domain (aa695-802). Additional isoforms exist resulting from an alternate start site at Met228 and a 23 aa substitution for aa649-911 that serves as a GPI-anchor motif. Over aa23-911, human Brevican shares 82% aa identity with mouse Brevican. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Inhibition. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml under non-reducing conditions only.., Immunoprecipitation: 25ug/ml immunoprecipitates recombinant human Brevican from conditioned cull culture medium., Inhibition (Blocking of receptor-ligand interaction): ~0.01-0.05ug/ml will block 50% of the binding of 1ug/ml of biotinylated Hyaluronan (132kD) to immobilized recombinant human Brevican (100ul of a 3ug/ml solution was coated in each well) in a functional ELISA. 1ug/ml will achieve >90% blocking in the assay., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Anti-BCAN, Anti-Brevican core protein, Anti-Chondroitin sulfate proteoglycan 7, Anti-Brain-enriched hyaluronan-binding protein |
Supplier: | United States Biological |
Supplier-Nr: | B2739-70B |
Properties
Application: | ELISA, IP, WB |
Antibody Type: | Monoclonal |
Clone: | 8H196 |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | NS0-derived rhBrevican. (aa 23 - 911) |
Format: | Purified |
Database Information
KEGG ID : | K06795 | Matching products |
UniProt ID : | Q96GW7 | Matching products |
Gene ID | GeneID 63827 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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