Anti-Aurora B

Human aurora A (aurora related kinase-1 (ARK-1), Aik1, STK15, BTAK, aurora2), and human aurora B (aurora related kinase-2 (ARK-2), Aik2, STK12, aurora1) are centrosome-associated serine/threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. Aurora B has unique genetic loci relative to Aurora A suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression. Aurora B exists in a complex with survivin and inner centromere protein (INCENP) in mitosis, and its kinase activity is stimulated by survivin binding and cell cycle-dependent phosphorylation. Phosphorylation of INCENP by aurora B stimulates aurora B kinase activity .5, Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence: 1-3ug/m, Western Blotting: 1-3ug/ml, Immunoprecipitation: 7ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.,