Anti-ATP1A4 / ATPase subunit alpha 4

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. ATP1A4 antibody detects Sodium/potassium-transporting ATPase subunit alpha-4, a testis-enriched isoform of the Na+/K+ ATPase that maintains ionic gradients across the plasma membrane. ATP1A4 catalyzes ATP hydrolysis to drive the active transport of sodium and potassium ions, sustaining electrochemical gradients essential for cell volume regulation, signal transduction, and sperm motility. The ATP1A4 antibody is widely used in reproductive biology, membrane physiology, and enzymology research to study ion homeostasis and energy metabolism.ATP1A4 is encoded by the ATP1A4 gene located on human chromosome 1p13.1. The protein is approximately 1,023 amino acids long and forms the catalytic alpha subunit of the Na+/K+ ATPase complex, which also includes a beta subunit and an FXYD regulatory protein. This enzyme maintains intracellular potassium and extracellular sodium concentrations by hydrolyzing ATP for ion exchange across the plasma membrane. The alpha-4 isoform is primarily expressed in spermatozoa and germ cells, where it contributes to flagellar motility and fertility.The ATP1A4 antibody detects a 112 kilodalton band by western blot and shows membrane localization in sperm and epithelial tissues under immunofluorescence microscopy. During spermatogenesis, ATP1A4 supports sperm maturation by regulating intracellular pH and calcium signaling. It localizes predominantly to the sperm plasma membrane, particularly the midpiece and flagellum, providing energy coupling for motility. Functional studies show that loss of ATP1A4 impairs sperm motility, reduces fertility, and alters ionic homeostasis in the reproductive tract.In somatic cells, ATP1A4 contributes to cellular polarization and signal transduction. Beyond ion transport, it functions as a signaling molecule, interacting with Src-family kinases and regulating MAPK pathways during sperm capacitation. This dual role as both a pump and signaling scaffold underscores its importance in reproductive physiology and beyond.Because of its testis-specific expression and regulatory versatility, ATP1A4 serves as a biomarker for sperm function, male fertility, and Na+/K+ ATPase diversity. NSJ Bioreagents provides a validated ATP1A4 antibody optimized for its applications, supporting studies in ion transport, signal regulation, and reproductive biology.