Anti-Annexin A2 (ANXA2, annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, annexin II,

Alternate Name: ANXA2, annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, annexin II, lipocortin II, Annexin II (lipocortin I), annexin II (lipocortin II), calpactin I, heavy polypeptide (p36), annexin II (lipocortin II calpactin I, heavy polypeptide), calpactin I heavy polypeptide, chromobindin 8, lipocortin II, placental anticoagulant protein IV, Domain Information: Annexin , Gene Ontology Terms: phospholipase inhibitor activity, calcium-dependent phospholipid binding, calcium ion binding, skeletal development, soluble fraction, plasma membrane, anticoagulant activity, Application(s): Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 35kD band observed in lysates of Human cell lines A549 and HeLa (calculated MW of 40.4kD according to NP_001002858.1 and 38.6kD according to NP_004030.1 and NP_001002857.1). Recommended concentration: 0.1-0.3ug/ml. Staining (0.1ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See A2295-15C-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.