Anti-Annexin A2 (Annexin II, ANX2, ANXA2, Calpactin 1 Heavy Chain, Lipocortin 2, p36, Placental Anti

Annexin A2 (ANXA2), also known as Annexin II and Lipocortin II (LPC2), is a 38.6kD member of the Annexin family of proteins. The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin's have a molecular weight of approximately 35-40kD and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C-terminal domain is comprised of four 60-70 amino acid (aa) repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly alpha-helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A2 was identified as an inhibitor of phospholipase A2 activity. Annexin A2 also functions as an autocrine factor to enhance osteoclast formation and bone resorption and is a major cellular substrate of the tyrosine kinase encoded by the SRC oncogene. Human Annexin A2 shares 97% identity with mouse and rat Annexin A2. , , Applications:, Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 5-15ug/ml, Optimal dilution to be determined by researcher., , Storage and Stability:, Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.