Anti-Akt3 (PKBgamma, RAC-PK Gamma), clone 4H307

Applications:, Suitable for use in Western Blot and Specificity Assay. Other applications not tested. Recommended Dilution: Western Blot: 0.1-1ug/ml detects Akt3/PKBg in RIPA lysates from HEK293 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Akt3/PKBg (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Specificity Assay: 1:5,000-1:20,000 dilutions were incubated with various recombinant Akt proteins conjugated to Luminex(R) microspheres. No crossreactivity with Akt1 or Akt2 was detected. Optimal dilutions to be determined by the researcher. , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.