Anti-Akt1 (Rac PKa, PKBa)

Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular , processes as glucose uptake, cell cycle progression, and apoptosis. Three highly , homologous members define the Akt family: Akt1 (PKBalpha), Akt2 (PKBbeta), and Akt3 , (PKBgamma). Akt1 is the most ubiquitously expressed family member. All three Akts contain , an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl- , terminal regulatory domain. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 50ug/ml. This antibody was validated for flow cytometry using MCF-7 cells. For , intracellular staining to detect Akt1, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 50ug/ml and add 10ul of the diluted solution to 1-5x10e5 cells in a total reaction volume not exceeding 20ulL. The binding of unlabeled monoclonal antibodies may be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. , Immunocytochemistry: 8-25ug/ml to detect Akt1 in fixed human peripheral blood mononuclear cells., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.