Anti-Adiponectin

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene which is physiologically active and specifically and highly expressed in adipose cells (adipokine)., The protein belongs to the soluble defence collagen superfamily. It has a collagen-like domain which is structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher-order complexes found circulating in serum. A reduction in adiponectin serum levels is accompanied by insulin resistance states such as obesity and type 2 diabetes mellitus. It is also reported in patients with coronary artery disease. Increased adiponectin levels are associated with type 1 diabetes mellitus, anorexia nervosa and chronic renal failure., Adiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation. It inhibits the inflammatory processes of atherosclerosis suppressing the expression of adhesion and cytokine molecules in vascular endothelial cells and macrophages, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Indirect ELISA: >1:100,000 using 2.5ng of antigen coated per well, Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.