Anti-A33 (GPA33, Glycoprotein A33 (transmembrane) Cell surface A33 antigen precursor, Glycoprotein A

Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214aa in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug stains 10e6 cells from the HT-29 human colon carcinoma cell line., Western Blot: 1ug/ml, Immunohistochemistry: 8-25ug/ml stains immersion fixed paraffin-embedded sections of human colon and human colon cancer tissue subjected to antigen retrieval, basic., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.