Anti-5-Lipoxygenase

5-Lipoxygenase (5-LO) is an enzyme encoded by ALOX5 in humans that is involved in leukotriene biosynthesis. It is comprised of an N-terminal regulatory domain and a C-terminal catalytic domain and is primarily expressed in leukocytes. Increases in intracellular calcium levels or cellular stress induce translocation of 5-LO from the cytosol or nucleoplasm, depending on the cell type and 5-LO phosphorylation status, to the nuclear envelope, where it interacts with 5-LO-activating protein (FLAP), which transfers arachidonic acid (Cay-90010, Cay-90010.1, Cay-10006607) to 5-LO. 5-LO catalyzes the conversion of arachidonic acid to 5(S)-HpETE (Cay-44230) and then to leukotriene A4 (LTA4). Other substrates of 5-LO include 5,8,11,14,17-eicosapentaenoic acid, 5,8,11-eicosatrienoic acid, 5,8-eicosadienoic acid, 12-HpETE, and 15-HpETE. Alox5 knockout mice are protected against arthritis and pulmonary inflammation. Knockout of Alox5 also protects apolipoprotein E-deficient hyperlipidemic mice from high-fat diet-induced hepatic injury and inflammation. Levels of 5-LO are elevated in postmortem hippocampus and cortex of patients with Alzheimer's disease. Cayman's 5-Lipoxygenase Polyclonal Antibody can be used for Western blot and immunohistochemistry applications. The antibody recognizes 5-LO at 78 kDa from human and porcine samples.Synonyms: ALOX5, Arachidonate 5-Lipoxygenase, 5-LO, LOG5, 5-LOX. Immunogen: Synthetic peptide from an internal region of human 5-lipoxygenase. Formulation: (Request formulation change), 500 µl of Peptide affinity-purified polyclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol, and 0.02% sodium azide. Host: Rabbit. Applications: IHC and WB. Cross Reactivity: (-) 12-Lipoxygenase, (-) 15-Lipoxygenase. UniProt Accession #: P09917. Origin: Animal/Rabbit.