VEGFR2 Extracellular Domain (human, recombinant, aa 20-327)

VEGFR2, also known as kinase insert domain receptor (KDR), is a receptor tyrosine kinase that has roles in vascular permeability, cell proliferation and survival, and angiogenesis. It is composed of an N-terminal extracellular ligand-binding domain, which contains seven immunoglobulin-like (Ig-like) domains, a transmembrane domain, and an intracellular tyrosine kinase domain, which is subject to phosphorylation and contains a kinase insert domain and the C-terminal domain. VEGFR2 is expressed primarily in vascular and lymphatic endothelial cells, where it is bound by the growth factors VEGF-A, VEGF-C, and VEGF-D. Upon ligand binding, VEGFR2 forms homodimers or heterodimers with VEGFR1 or VEGFR3, resulting in VEGFR2 phosphorylation and activation of a variety of intracellular signaling pathways, including the ERK/MAPK pathway, to promote endothelial cell survival, proliferation, and migration. VEGFR2 also exists in a soluble form that is generated by alternative splicing of the KDR pre-mRNA or proteolytic shedding and decreases angiogenesis by functioning as a decoy receptor for VEGF-A. VEGFR2 expression is increased in the tumor vasculature of patients with a variety of solid tumors, including colorectal, lung, pancreatic, and breast cancer. Cayman's VEGFR2 Extracellular Domain (human, recombinant, aa 20-327) protein can be used for enzyme activity assays. This protein is a disulfide-linked homodimer. The reduced monomer, comprised of VEGFR2 (amino acids 20-327) fused to human IgG1 Fc at its C-terminus, consists of 549 amino acids, has a calculated molecular weight of 61.5 kDa, and a predicted N terminus of Ala20 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at greater than 61.5 kDa by SDS-PAGE under reducing conditions.Synonyms: CD309, Fetal Liver Kinase 1, FLK1, FLK-1, KDR, Kinase Insert Domain Receptor, Vascular Endothelial Growth Factor Receptor 2. Purity: >95% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg determined by the LAL endotoxin assay. Source: Active recombinant C-terminal human IgG1 Fc-tagged VEGFR2 expressed in HEK293 cells. Amino Acids: 20-327. MW: 61.5 kDa. Formulation: (Request formulation change), Lyophilized from sterile PBS, pH 7.4. Host: HEK293 cells. UniProt Accession #: P35968.