Phosphodiesterase 1A, Recombinant, Human (PDE1A)

Phosphodiesterase 1A, Recombinant, Human (PDE1A)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
P4072A.10 10 µg - -

3 - 19 business days*

1,106.00€
 
Human PDE1A (GenBank Accession No. NM_005019), full length with N-terminal GST tag, expressed in... more
Product information "Phosphodiesterase 1A, Recombinant, Human (PDE1A)"
Human PDE1A (GenBank Accession No. NM_005019), full length with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 123 U/ug, 1 unit will lease 1nmole phosphate per minute from 400uM 5'- AMP at 30°C in a reaction buffer of 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2. Assay Buffer: 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2, 0.2mM CaCl2, and 240nM Calmodulin, 3', 5'-cAMP substrate: 0.4mM, PDE1A: 1ng/ul. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Keywords: hCam-1, Cam-PDE 1A, EC=3.1.4.17, 61 kDa Cam-PDE, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A
Supplier: United States Biological
Supplier-Nr: P4072A

Properties

Application: Enzyme assays
Conjugate: No
Format: Purified

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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