PDE9A2 (human, recombinant)

Phosphodiesterase 9A (PDE9A) is a high-affinity cGMP-specific cyclic nucleotide phosphodiesterase. Alternative splicing in the 5' region of PDE9A mRNA results in a variety of PDE9A isoforms, including PDE9A2, whereas the 3' region encoding the catalytic PDE domain does not vary between isoforms. PDE9A2 mRNA is expressed in colon, prostate, spleen, peripheral blood leukocytes, small intestine, thymus, testis, and ovary. PDE9A2 localizes to membrane ruffles, the perinuclear region, and other membrane regions when transiently overexpressed in HeLa and COS-1 cells. PDE9A expression is elevated in reticulocytes and neutrophils isolated from patients with sickle cell disease compared with healthy individuals. Cayman's PDE9A2 (human, recombinant) protein consists of 770 amino acids and has a calculated molecular weight of 89.5 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 75 kDa.Synonyms: High Affinity cGMP-specific 3',5'-cyclic Phosphodiesterase 9A. Purity: >90% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg, determined by the LAL endotoxin assay. Source: Recombinant human N-terminal His-GST-tagged PDE9A2 expressed in insect cells. Amino Acids: 1-533 (full length). MW: 89.5 kDa. Formulation: (Request formulation change), Lyophilized from sterile 20 mM Tris, pH 7.4, with 500 mM sodium chloride, 5 mM GSH, and 10% glycerol. UniProt Accession #: O76083-2.