MeCP2 (human recombinant, methyl binding domain aa 77-166)

DNA methylation occurs mainly at the 5'-position of cytosine rings (5-methylcytosine (5mC)). The 5mC bases are found largely in genomic regions with a high frequency of cytosines and guanines, called CpG islands. Methyl-CpG binding protein 2 (MeCP2) specifically binds to methylated promoters on CpG islands and mediates gene silencing by recruiting corepressor complexes. The Methyl-CpG Binding Domain (MBD) protein family consists of five members: MeCP2, MBD1, MBD2, MBD3 and MBD4. The sequence similarity between the MBD proteins is limited to the MBD domains. In vitro work suggests high affinity binding of MeCP2 is facilitated by DNA fragments containing A/T bases ([A/T]>4) adjacent to the methyl-CpG. Mutations in the MeCP2 gene have been linked to the X-linked neurological disorder, Rett Syndrome.Synonyms: Methyl-CpG Binding Protein 2. Purity: >95%. Source: Recombinant N-terminal GST-tagged protein expressed in E. coli. Amino Acids: 77-166 (partial protein). MW: 37 kDa. Formulation: (Request formulation change), 50 mM Tris, pH 7.5, with 500 mM sodium chloride, 5% glycerol, and 5 mM beta-mercaptoethanol. UniProt Accession #: P51608.