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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
Cay40373-100 | 100 µg | - | - |
6 - 10 business days* |
448.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
K-Ras is a small GTPase and member of the RAS family of GTPases with roles in apoptosis, as well... more
Product information "K-Ras(G12V) Isoform B (human, recombinant)"
K-Ras is a small GTPase and member of the RAS family of GTPases with roles in apoptosis, as well as cell proliferation, survival, and migration. K-Ras is composed of a guanine nucleotide-binding domain containing an active site, an effector binding domain, and an isoform-specific C-terminal hypervariable region (HVR) that differs between K-Ras isoforms A and B due to alternative splicing. The active site cycles between GDP-bound inactive and GTP-bound active states and is regulated by its associations with GTPase-activating proteins (GAPs) or guanine nucleotide exchange factors (GEFs). K-Ras isoform B is ubiquitously expressed and is tethered to the intracellular side of cell membranes via farnesyl lipidation and to negatively charged regions of the membrane via two positively charged polybasic regions in the HVR. K-Ras(G12V), which contains a glycine-to-valine substitution at position 12, is constitutively active and associated with pancreatic, colon, and lung cancers. Tumor levels of K-Ras(G12V) are negatively correlated with overall survival in patients with colorectal cancer. Cayman's K-Ras(G12V) Isoform B (human, recombinant) protein consists of 184 amino acids, has a calculated molecular weight of 22 kDa, and can be used for enzyme activity assay, inhibitor screening, and inhibitor selectivity profiling applications.Synonyms: c-K-ras(G12V), c-Ki-ras(G12V), K-Ras4A(G12V), Ki-Ras(G12V), Kirsten Rat Sarcoma Virus(G12V). Purity: >53% estimated by SDS-PAGE. Source: Recombinant human N-terminal His-tagged K-Ras(G12V) isoform B expressed in insect cells. Amino Acids: 2-185. MW: 22 kDa. Formulation: (Request formulation change), 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, 0.04% Tween20, 20% glycerol, 3 mM DTT, and 120 mM imidazole. UniProt Accession #: P01116.
Keywords: | KRAS, KRAS2, Ki-Ras, K-Ras 2, c-K-ras, c-Ki-ras, GTPase KRas, GTPase KRas, N-terminally processed |
Supplier: | Cayman Chemical |
Supplier-Nr: | 40373 |
Properties
Application: | Active |
Conjugate: | No |
Host: | Insect cells |
Species reactivity: | human |
MW: | 22 kD |
Purity: | >53% estimated by SDS-PAGE |
Format: | Solution |
Database Information
KEGG ID : | K07827 | Matching products |
UniProt ID : | P01116 | Matching products |
Gene ID | GeneID 3845 | Matching products |
Handling & Safety
Storage: | -80°C |
Shipping: | -80°C (International: -80°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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