K-Ras(G12C) Isoform A (human, recombinant)

K-Ras is a small GTPase and member of the RAS family of GTPases with roles in apoptosis, as well as cell proliferation, survival, and migration. K-Ras is composed of a guanine nucleotide binding domain containing an active site, an effector binding domain, and an isoform-specific C-terminal hypervariable region, which varies by four amino acids between isoforms A and B. The active site cycles between GDP-bound inactive and GTP-bound active states and is regulated by its associations with GTPase-activating proteins (GAPs) or guanine nucleotide exchange factors (GEFs). K-Ras is ubiquitously expressed and is tethered to the intracellular side of cell membranes via farnesyl and palmitoyl lipidation. The glycine-to-cysteine substitution at position 12 mutant K-Ras (K-Ras(G12C)) is constitutively active and found in pancreatic, colon, and lung cancers. Inhibition of K-Ras(G12C) with a cysteine-targeted inhibitor ARS1620 (Cay-27915) reduces tumor volume in K-Ras(G12C)-expressing, but not K-Ras(G12V)-expressing, MiaPaCa-2 pancreatic cancer mouse xenograft models. Tumor levels of K-Ras(G12C) are increased in patients with lung adenocarcinoma who reported to be former or current smokers. Cayman's K-Ras(G12C) Isoform A (human, recombinant) consists of 185 amino acids and has a calculated molecular weight of 23 kDa. By SDS-PAGE, the apparent molecular mass of the protein is 25-26 kDa due to glycosylation.Synonyms: c-K-ras(G12C), K-Ras4A(G12C), c-Ki-ras(G12C), Ki-Ras(G12C), Kirsten Rat Sarcoma Virus(G12C), KRAS(G12C). Purity: >88% estimated by SDS-PAGE. Source: Recombinant human N-terminal His-tagged K-Ras(G12C) isoform A expressed in E. coli. Amino Acids: 2-186. MW: 23 kDa. Formulation: (Request formulation change), 20 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM DTT. UniProt Accession #: P01116.