JMJD2D Strep-tagged (human recombinant)

Methylation of lysine residues in core histones plays a critical role in regulating gene expression. Jumonji domain containing 2D (JMJD2D) catalyzes the demethylation of di- and tri-methylated forms of histone H3 at lysine residue 9 (me 2/3), leading to transcriptional repression and activation, respectively. Like other JmjC protein hydroxylase family members, JMJD2D is an alpha-ketoglutarate-dependent Fe (II) oxygenase. Purification of Fe-dependent JmjC family members by IMAC can result in displacement of the catalytic iron and decreased activity, therefore this Strep-tagged protein is purified by affinity chromatography using Strep-Tactin coated resin. Because of their implication in cancer cell growth, jumonji C domain-containing histone demethylase inhibitors may have the capacity to be anticancer agents.Synonyms: Jumonji Domain Containing 2D, KDM4D, Lysine-specific Demethylase 4D. Purity: >70% estimated by SDS-PAGE. Source: active recombinant N-terminal Strep II-tagged protein expressed in E. coli. Amino Acids: 4-354. MW: 42.6 kDa. Formulation: (Request formulation change), 20 mM HEPES, pH 7.4, and 150 mM sodium chloride. License: Sold under license from IBA.. UniProt Accession #: Q6B0I6.