IDO (mouse, rec.) (His)

Mouse IDO (amino acids 1-407) is fused at the C-terminus to a His-tag. Specific Activity: >100'000U/mg protein with L-tryptophan as substrate. One unit is defined as the amount of enzyme that produces 1nmol of kynurenine per hour. Liquid. 0.2µm-filtered solution in 50mM TRIS-Cl, pH 7.4, containing 1mM EDTA. IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.