GPX4 (human, recombinant, His-tagged)

Glutathione peroxidase 4 (GPX4) is a selenocysteine-containing glutathione peroxidase that is encoded by the GPX4 gene in humans and protects cellular membranes from oxidative damage. It is a monomeric protein consisting of a thioredoxin motif and a selenocysteine-glutamine-tryptophan catalytic triad that reduces lipid hydroperoxides, including phospholipid, polyunsaturated lipid, and sterol hydroperoxides, to non-toxic lipid alcohols. During this process, the active site selenocysteine becomes oxidized and must subsequently be replenished by the reducing substrate glutathione (GSH). There are three isoforms of GPX4, mitochondrial mGPX4, cytosolic cGPX4, and nuclear nGPX4/snGPX4, that are expressed in all tissue types in rats, with the highest mRNA levels observed in testes. GPX4 is a key regulator of ferroptosis that inhibits ferroptotic cell death by preventing iron-dependent accumulation of toxic lipid reactive oxygen species. Mutations in GPX4 have been found in patients with Sedaghatian-type spondylometaphyseal dysplasia (SSMD), and silencing of Gpx4 in mice is embryonic lethal. Cayman's GPX4 (human, recombinant, His-tagged) protein has a selenocysteine incorporated in the active site that has been confirmed by mass spectrometry. It can be used for Western blot, ELISA, and enzymatic assays.Synonyms: Glutathione Peroxidase 4, PHGPx, Phospholipid Hydroperoxide Glutathione Peroxidase. Purity: >65% estimated by SDS-PAGE. Protein Identity Confirmed by Mass Spec: Yes. Source: Active recombinant N-terminal His-tagged protein expressed in E. coli strain C321.DeltaA.exp. Amino Acids: 1-170 (full length). MW: 21.2 kDa. Formulation: (Request formulation change), 50 mM potassium phosphate buffer, pH 7.6, with 0.1 mM DTT and 5% glycerol. UniProt Accession #: P36969-2.