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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
T9160-15P1.5 | 5 µg | - | - |
3 - 19 business days* |
598.00€
|
||
T9160-15P1.20 | 20 µg | - | - |
3 - 19 business days* |
757.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their... more
Product information "Tumor Necrosis Factor alpha (TNFa), porcine recombinant (rpTNFa)"
TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS . Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2 , GM-CSF. Description: Recombinant Porcine TNF-a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17,274 Dalton. Porcine TNF-alpha is purified by standard chromatographic techniques. Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser. Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: Porcine TNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytotoxicity of porcine PK15 cells was found to be < 0.008ng/ml. Endotoxin: < 0.1ng/ug (IEU/ug) of Porcine TNF-alpha. Protein Content: Protein quantitation was carried out by two independent methods:, 1. UV spectroscopy at 280 nm using the absorbency value of 1.26 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics)., 2. Analysis by RP-HPLC, using a calibrated solution of TNF-alpa as a Reference Standard. Solubility: It is recommended to reconstitute the lyophilized Porcine TNF-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized Porcine TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution TNF-alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Keywords: | swine |
Supplier: | United States Biological |
Supplier-Nr: | T9160-15P1 |
Properties
Conjugate: | No |
Host: | E.coli |
Species reactivity: | swine |
Format: | Highly Purified |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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