Interleukin-13/IL-13 Protein(Ser26-Phe131,C-6His) (recombinant mouse)

Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cellsThe ED50 for this effect is less than 5 ng/ml. Protein Construction: Recombinant Mouse Interleukin-13 is produced by our Mammalian expression system and the target gene encoding Ser26-Phe131 is expressed with a 6His tag at the C-terminus. Sequence: Ser26-Phe131. Fusion tag: C-6His Endotoxin: < 1.0 EU per µg as determined by the LAL method. Apparent Molecular Mass: 15-30 kDa. Protein function: Mouse interleukin 13 (mIL-13) is a pleiotropic cytokine produced by activated Th2 cells. IL-13 induces B cell proliferation and immunoglobin production. It contains a four helical bundle with two internal disulfide bonds. Mouse IL13 shares 58% sequence identity with human protein and exhibits cross-species activity. IL13 signals via receptor IL13R (type2, IL4R) and activates STAT-6. IL13 initially binds IL-13Ra1 with low affinity and triggers association of IL4Ra, generating a high affinity heterodimeric receptor IL13R and eliciting downstream signals. IL13 also binds IL-13Ra2 with high affinity, which plays a role in a negative feedback system of IL13 signaling. IL13 is an important mediator of allergic inflammation and disease. Protein function: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (PubMed:25847241). [The UniProt Consortium]