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Zymostenol is a late-stage precursor in the biosynthesis of cholesterol. It is an agonist of retinoic acid receptor-related orphan receptor gamma (RORgamma) with an EC50 value of 1 µM in a RORgamma coactivator recruitment assay in the presence of ursolic acid (Cay-10072). It increases the number of myelin basic protein-positive oligodendrocytes generated from oligodendrocyte precursor cells in vitro. Zymostenol accumulates in cells following administration of microsomal antiestrogen-binding site (AEBS) ligands, such as tamoxifen (Cay-13258), which are associated with cell differentiation and a protective type of autophagy. When used alone at a concentration of 20 µM, zymostenol halts the cell cycle at the G0/G1 phase and increases the levels of free sterols, esterified sterols, and triacylglycerols in MCF-7 cells.Formal Name: (5alpha)-cholest-8-en-3beta-ol. CAS Number: 566-97-2. Synonyms: Delta8-Cholesterol. Molecular Formula: C27H46O. Formula Weight: 386.7. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 3 mg/ml, Ethanol: 2 mg/ml. SMILES: C[C@H](CCCC(C)C)[C@@]1([H])CC[C@@]([C@]1(C)CC2)([H])C3=C2[C@]4(C)[C@](CC3)([H])C[C@@H](O)CC4. InChi Code: InChI=1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h18-21,23-24,28H,6-17H2,1-5H3/t19-,20+,21+,23-,24+,26+,27-/m1/s1. InChi Key: QETLKNDKQOXZRP-XTGBIJOFSA-N.
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