Tolfenamic Acid

Tolfenamic acid is a non-steroidal anti-inflammatory drug (NSAID) with anticancer activity. It is selective for COX-2 over COX-1 in canine DH82 monocyte/macrophage cells (IC50s = 3.53 and >51.2 µg/ml, respectively). Tolfenamic acid inhibits calcium influx in human polymorphonuclear leukocytes (PMNLs) induced by N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP, Cay-21495) or the calcium ionophore A23187 (Cay-11016, Cay-22030) in a concentration-dependent manner. It decreases protein levels of the transcription factors Sp1, Sp3, and Sp4 in PANC-1 and L3.6pl cells when used at a concentration of 50 µM and inhibits proliferation of PANC-1, L3.6pl, and PANC-28 cells in a concentration-dependent manner. Tolfenamic acid (50 and 100 µM) decreases the viability of and induces apoptosis in MDA-MB-231 cells. It reduces tumor growth in an MDA-MB-231 mouse xenograft model when administered at doses of 25 and 50 mg/kg. Tolfenamic acid (150 µmol/kg) reduces carrageenan-induced paw edema in mice by 24%.Formal Name: 2-[(3-chloro-2-methylphenyl)amino]-benzoic acid. CAS Number: 13710-19-5. Molecular Formula: C14H12ClNO2. Formula Weight: 261.7. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: acidic PBS: 50 mg/ml, basic PBS: 50 µg/ml, DMF: 59 mg/ml, DMSO: 39 mg/ml, Ethanol: 11.5 mg/ml, PBS pH 7.0: 50 µg/ml. SMILES: OC(=O)c1ccccc1Nc1cccc(Cl)c1C. InChi Code: InChI=1S/C14H12ClNO2/c1-9-11(15)6-4-8-12(9)16-13-7-3-2-5-10(13)14(17)18/h2-8,16H,1H3,(H,17,18). InChi Key: YEZNLOUZAIOMLT-UHFFFAOYSA-N.