Longdaysin

Longdaysin is an inhibitor of casein kinase 1delta (CK1delta) and CK1alpha (IC50 = 12 µM for both). It also inhibits cyclin-dependent kinase 2 (Cdk2), Cdk7, MAPK, and MAP3K (IC50s = 13, 32, 59, and 92 µM, respectively). Longdaysin increases the circadian period of U2OS cells by one and five hours when used at concentrations of 0.39 and 3.4 µM, respectively. It inhibits Wnt-signaling induced by Wnt3A-containing conditioned medium in HEK293 cells and reduces levels of active beta-catenin in HEK293 cells expressing CK1delta or CK1epsilon. Longdaysin (10 and 25 µM) reduces colony formation, as well as cell migration and invasion, by Hs 578T and MDA-MB-231 breast cancer cells. In vivo, longdaysin (5 mg/kg) reduces tumor volume in an MDA-MB-231 mouse xenograft model.Formal Name: 9-(1-methylethyl)-N-[[3-(trifluoromethyl)phenyl]methyl]-9H-purin-6-amine. CAS Number: 1353867-91-0. Molecular Formula: C16H16F3N5. Formula Weight: 335.3. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 30 mg/ml, DMSO: 20 mg/ml, Ethanol: 10 mg/ml. lambdamax: 211, 273 nm. SMILES: FC(F)(C1=CC(CNC2=NC=NC3=C2N=CN3C(C)C)=CC=C1)F. InChi Code: InChI=1S/C16H16F3N5/c1-10(2)24-9-23-13-14(21-8-22-15(13)24)20-7-11-4-3-5-12(6-11)16(17,18)19/h3-6,8-10H,7H2,1-2H3,(H,20,21,22). InChi Key: REKSFCCYDQMSIN-UHFFFAOYSA-N.