Lin28 1632

Lin28 1632 is a small molecule inhibitor of the interaction between the RNA binding protein Lin28 and let-7 precursor RNA (IC50 = 8 µM in a competition ELISA). It increases levels of endogenous let-7 miRNAs and decreases Lin28 expression in a dose-dependent manner in murine embryonic stem cells (mESCs). Lin28 1632 inhibits clonogenic growth of 22Rv1, PC3, DU145, and Huh7 cancer cells and decreases tumor-sphere formation by 22Rv1 and Huh7 cells in vitro. It also binds bromodomain-containing protein 4 (BRD4) and CREB-binding protein (CBP/CREBBP) bromodomains (Kds = 7 and 25 µM, respectively).Formal Name: N-methyl-N-[3-(3-methyl-1,2,4-triazolo[4,3-b]pyridazin-6-yl)phenyl]-acetamide. CAS Number: 108825-65-6. Molecular Formula: C15H15N5O. Formula Weight: 281.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: 30 mg/ml, PBS (pH 7.2): 0.5 mg/ml. lambdamax: 247, 285 nm. SMILES: CN(C(C)=O)C1=CC=CC(C(C=C2)=NN3C2=NN=C3C)=C1. InChi Code: InChI=1S/C15H15N5O/c1-10-16-17-15-8-7-14(18-20(10)15)12-5-4-6-13(9-12)19(3)11(2)21/h4-9H,1-3H3. InChi Key: WPAQLESUVYGUJZ-UHFFFAOYSA-N.