JAK Inhibitor 31

JAK inhibitor 31 is an inhibitor of JAK2 (IC50 = 3.9 nM). It is selective for JAK2 over JAK3 (IC50s = 70 nM), as well as Aurora B kinase, VEGFR2, Abl1, and GSK3beta (IC50s = 0.441, 1.5, 21.37, and 50 µM, respectively), but does inhibit JAK1 and TYK2 (IC50s = 7 and 2.9 nM, respectively). JAK inhibitor 31 inhibits STAT3-mediated transcription of IL6 in a secreted alkaline phosphatase (SEAP) reporter assay (IC50 = 162 nM). It reduces ocular inflammation, as well as decreases Il6 expression in the meibomian glands, in a mouse model of allergic eye disease when used at concentrations of 1 or 3 mg/ml.Formal Name: 1-[5-methyl-2-[(3-methyl-5-isothiazolyl)amino]-4-pyrimidinyl]-3-(4-methyl-1-piperazinyl)-3-azetidineacetonitrile. CAS Number: 2891469-99-9. Synonyms: Janus-Associated Kinase Inhibitor 31. Molecular Formula: C19H26N8S. Formula Weight: 398.5. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, Ethanol: 10 mg/ml, PBS (pH 7.2): Slightly soluble. lambdamax: 226, 254, 286 nm. SMILES: CC1=C(N2CC(N3CCN(C)CC3)(CC#N)C2)N=C(NC4=CC(C)=NS4)N=C1. InChi Code: InChI=1S/C19H26N8S/c1-14-11-21-18(22-16-10-15(2)24-28-16)23-17(14)26-12-19(13-26,4-5-20)27-8-6-25(3)7-9-27/h10-11H,4,6-9,12-13H2,1-3H3,(H,21,22,23). InChi Key: VGBYAYCYPVFDTM-UHFFFAOYSA-N.