C18

C18 is a covalent inhibitor of glutathione peroxidase 4 (GPX4, IC50 = 120 nM). It inhibits the growth of MDA-MB-468, BT-549, and MDA-MB-231 breast cancer cells (IC50s = 10, 75, and 12 nM, respectively). C18 (10 and 20 µM) increases lipid peroxidation and oxidized malondialdehyde (MDA) levels in MDA-MB-231 cells, an effect that can be prevented by the ferroptosis inhibitor ferrostatin-1 (Cay-17729). It decreases mitochondrial volume and induces degradation of mitochondrial crests and outer membranes in MDA-MB-231 cells when used at a concentration of 20 µM. In vivo, C18 (5, 10, or 20 mg/kg) reduces tumor volume and weight, without affecting body weight, in an MDA-MB-231 mouse xenograft model.Formal Name: 2-chloro-N-(2,3-dihydro-1,4-benzodioxin-6-yl)-N-(4-fluoro-3,5-dimethoxyphenyl)-acetamide. CAS Number: 2922824-09-5. Molecular Formula: C18H17ClFNO5. Formula Weight: 381.8. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 1 mg/ml, DMSO: 1 mg/ml, Ethanol: Slightly Soluble. SMILES: O=C(CCl)N(C1=CC(OC)=C(C(OC)=C1)F)C2=CC=C3OCCOC3=C2. InChi Code: InChI=1S/C18H17ClFNO5/c1-23-15-8-12(9-16(24-2)18(15)20)21(17(22)10-19)11-3-4-13-14(7-11)26-6-5-25-13/h3-4,7-9H,5-6,10H2,1-2H3. InChi Key: HKXSBJJCHBFTMB-UHFFFAOYSA-N.