Aminopurvalanol A

Aminopurvalanol A is a selective, cell-permeable, and competitive cyclin-dependent kinase (CDK) inhibitor that potently inhibits Cdk1/cyclin B, Cdk2/cyclin A, Cdk2/cyclin E, and Cdk5/p35 (IC50s = 33, 33, 28, and 20 nM, respectively). It is over 90-fold selective for CDKs over ERK1, ERK2, PKC-delta, protein kinase A (PKA), casein kinase 1, insulin receptor tyrosine kinase (IC50s = 3.0-36 µM), and over 3,000-fold selective over a range of other kinases (IC50s > 100 µM). In antiproliferative assays in vitro, aminopurvalanol A inhibits growth of ovarian (IGROV1), leukemic (SR), lung (NCI-H522), and colonic (KM12) cells (GI50s = 470, 420, 1,000, and 30 nM, respectively). Aminopurvalanol A also inhibits mitotic division and preferentially arrests cells in the G2/M phase via Cdk1/cyclin BFormal Name: (2R)-2-[[6-[(3-amino-5-chlorophenyl)amino]-9-(1-methylethyl)-9H-purin-2-yl]amino]-3-methyl-1-butanol. CAS Number: 220792-57-4. Synonyms: NG 97. Molecular Formula: C19H26ClN7O. Formula Weight: 403.9. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 50 mg/ml, DMF:PBS (pH 7.2) (1:5): 0.2 mg/ml, DMSO: 30 mg/ml, Ethanol: 10 mg/ml. lambdamax: 211, 256, 315 nm. SMILES: CC(C)N1C2=NC(N[C@H](C(C)C)CO)=NC(NC3=CC(Cl)=CC(N)=C3)=C2N=C1. InChi Code: InChI=1S/C19H26ClN7O/c1-10(2)15(8-28)24-19-25-17(23-14-6-12(20)5-13(21)7-14)16-18(26-19)27(9-22-16)11(3)4/h5-7,9-11,15,28H,8,21H2,1-4H3,(H2,23,24,25,26)/t15-/m0/s1. InChi Key: RAMROQQYRRQPDL-HNNXBMFYSA-N.