2-Acetylphenothiazine

2-Acetylphenothiazine (2-APT) is a selective, cell-active inhibitor of NADPH oxidase 1 (NOX1) that blocks the generation of reactive oxygen species (ROS) in HT-29 cells with an IC50 value of 0.129 µM. It does not affect xanthine oxidase-dependent or mitochondrial ROS generation. 2-APT prevents ROS-dependent formation of ECM-degrading invadopodia in colon cancer cells. It also abolishes collagen-induced superoxide production by platelets (IC50 = 306 nM), preventing platelet aggregation and thrombus formation. 2-APT protects beta cells from cytokine-induced apoptosis by inhibiting NOX1. 2-APT can also activate the human transient receptor potential ankyrin 1 (TRPA1) nociceptor at 1-30 µM.Formal Name: 1-(10H-phenothiazin-2-yl)-ethanone. CAS Number: 6631-94-3. Synonyms: 2-APT, ML-171, NSC 57951, NSC 169669. Molecular Formula: C14H11NOS. Formula Weight: 241.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 25 mg/ml, DMF:PBS (pH 7.2) (1:4): 0.2 mg/ml, DMSO: 20 mg/ml, Ethanol: 1 mg/ml. lambdamax: 245, 282 nm. SMILES: O=C(C)C1=CC2=C(C=C1)SC3=CC=CC=C3N2. InChi Code: InChI=1S/C14H11NOS/c1-9(16)10-6-7-14-12(8-10)15-11-4-2-3-5-13(11)17-14/h2-8,15H,1H3. InChi Key: JWGBOHJGWOPYCL-UHFFFAOYSA-N.