Interleukin 2 Receptor alpha, Soluble, Human (IL-2 sRa) BioAssay(TM) ELISA Kit

This sandwich ELISA recognizes both natural and recombinant human IL-2 sRa. This kit exhibits no significant cross-reactivity with factors related to or associated with IL-2 sRa such as rhIL-2, rhIL-2 sRb. No significant cross-reactivity was observed with recombinant human: IL-1a, IL-1b, IL-1 sRI, IL-1 sRII, IL-1ra, IL-3, IL-3 sR a, IL-5, IL-5 sR a, IL-5 sRb, IL-6, IL-6 sR, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13 ANG, AR, CNTF, b-ECGR, EGF, EPO, FGF acidic, FGF basic, FGF-4, FGF-5, FGF-6, FGF-7, G-CSF, GM-CSF, sgp 130, GRO a, GROb, GROg, HB-EGF, HGF, INF-g, IGF-I IGF-II, LAP (TGF-b1), LIF, M-CSF, MCP-1, MIP-1a, MIP-1b, b-NGF, OSN, PD-ECGF, PDGF-AA, PDGF-AB, PDGF-BB, PTN, RANTES, SCF, SLPI, TGF- a, TGF-b, TNF- a, TNF-b, sTNF RI, sTNF RII, VEGF. Sensitivity: 10pg/ml , Range: , 62.5-2000pg/ml, Kit Components: I7663-31B1: Microtiter Plate 1x96 wells, I7663-31B2: Pab (Biotin) 1x6ml, I7663-31B3: Avidin (HRP) 1x12ml, I7663-31B4: Standard (4000pg) 2x1vial, I7663-31B5: Calibrator Diluent I 1x25ml. For serum/plasma testing., I7663-31B6: Calibrator Diluent II 1x25ml. For cell culture supernatant testing., I7663-31B7: Wash Buffer (20X) 1x60ml, I7663-31B8: Substrate A 1x10ml. Buffered solution with H2O2, I7663-31B9: Substrate B 1x10ml. Buffered solution with TMB., I7663-31B10: Stop Solution 1x14ml. 2N Sulphuric Acid (H2SO4). , Storage and Stability: Store at 4°C. Store I7663-31B4 powder at 4°C liquid at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.