Collagen Type IV Alpha 5 (COL4a5) BioAssay(TM) ELISA Kit (Human)

This sandwich ELISA kit is used for in vitro quantitative measurement of COL4a5 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Sensitivity: 1.12ng/ml, Range: 3.12-200ng/ml, Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type IV Alpha 5 (COL4a5). No significant cross-reactivity or interference between Collagen Type IV Alpha 5 (COL4a5) and analogues was observed. Precision: Intra-Assay: CV<10% , Inter-Assay: CV<12%, Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to COL4a5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to COL4a5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain COL4a5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of COL4a5 in the samples is then determined by comparing the O.D. of the samples to the standard curve. Kit Components: 96-well strip plate, 1x Plate , Standard, 2x vial, Detection Reagent A, 1×120ul, Detection Reagent B, 1×120ul, TMB Substrate, 1×9ml, Wash Buffer (30X), 1×20ml, Standard Diluent, 1×20ml, Assay Diluent A, 1×12ml, Assay Diluent B, 1×12ml, Stop Solution, 1x6ml, Storage and Stability: Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.