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| Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
|---|---|---|---|---|---|---|---|
| BPS-82179 | 384 rxns | - | - |
3 - 15 business days* |
2,518.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The MDM2-Driven p53 Ubiquitination Assay kit is a sensitive AlphaLisa(R) high-throughput... more
Product information "MDM2-Driven p53 Ubiquitination Assay Kit"
The MDM2-Driven p53 Ubiquitination Assay kit is a sensitive AlphaLisa(R) high-throughput screening (HTS) assay designed to measure MDM2 (mouse double minute 2 homolog) E3 ligase activity in a homogeneous 384 reaction format. The assay kit comes with enough biotinylated ubiquitin, ATP, FLAG-tagged p53, assay buffer, detection buffer, purified UBE1 (E1), UbcH5b (E2), and MDM2 (E3) for 384 reactions. The assay can detect mono-ubiquitination and poly-ubiquitination of p53. E1 and E2 enzymes are incubated with the E3 complex and FLAG-tagged p53, in the presence of biotin-conjugated ubiquitin and ATP. Ubiquitination of p53 occurs in a multistep ubiquitin transfer from E1 to E2 to E3, and E3-mediated conjugation of ubiquitin to p53. Next, acceptor beads are added, followed by streptavidin-conjugated donor beads. Alpha-counts are then measured. The increase in Alpha-counts is proportional to the mono- or poly-ubiquitination of the FLAG-tagged p53.
| Keywords: | Hdm2, MDM2, Oncoprotein Mdm2, Double minute 2 protein, p53-binding protein Mdm2, E3 ubiquitin-protein ligase Mdm2, RING-type E3 ubiquitin transferase Mdm2, |
| Supplier: | BPS Bioscience |
| Supplier-Nr: | 82179 |
Properties
| Application: | Inhibitor screening, drug discovery, HTS, IC50 determination, MDM2-driven ubiquitination studies |
| Species reactivity: | human |
Database Information
| KEGG ID : | K06643 | Matching products |
| UniProt ID : | Q00987 | Matching products |
| Gene ID | GeneID 4193 | Matching products |
Handling & Safety
| Storage: | -80°C |
| Shipping: | -80°C (International: °C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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