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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
E-BC-F050.96 | 96 wells | - | - |
7 - 16 business days* |
356.00€
|
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You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Detection principle: Arachidonic acid generates prostaglandins under the catalysis of... more
Product information "Cyclooxygenase (COX) Activity Fluorometric Assay Kit"
Detection principle: Arachidonic acid generates prostaglandins under the catalysis of cyclooxygenase, and under the action of cofactor (heme), cyclooxygenase activates peroxidase activity, converting fluorescent red into fluorescent substance. Performance characteristics: Sample type: animal tissue Sensitivity: 0.03 U/L Detection range: 0.03-9.66 U/L Detection method: Fluorescence method Assay type: Enzyme Activity Assay time: 70 min Precision: Average inter-assay CV: 5.0-7.0%Average intra-assay CV: 3.5-5.0% Other reagents required: Normal saline (0.9% NaCl) Storage: -20? Valid period: 12 months. COX-1 Protein function: Dual cyclooxygenase and peroxidase that plays an important role in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)- eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons (PubMed:7947975). Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells (Probable). Can also use linoleate (LA, (9Z,12Z)- octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)- HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products. [The UniProt Consortium]
Keywords: | PHS 1, COX-1, PGHS-1, PGH synthase 1, Cyclooxygenase-1, Prostaglandin H2 synthase 1, Prostaglandin G/H synthase 1, Prostaglandin-endoperoxide synthase 1, Cyclooxygenase (COX) Activity Fluorometric Assay Kit |
Supplier: | Elabscience |
Supplier-Nr: | E-BC-F050 |
Properties
Database Information
KEGG ID : | K00509 | Matching products |
UniProt ID : | P23219 | Matching products |
Gene ID | GeneID 5742 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | -20°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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