Anti-STAT3alpha/beta (Phospho-Tyr705) Rabbit Monoclonal Antibody (Clone RM261)

STAT3 is a ubiquitously expressed transcription factor and member of the STAT protein family that has roles in a variety of cellular functions including proliferation, apoptosis, and differentiation, as well as innate and adaptive immunity and stem cell pluripotency. It contains N-terminal, coiled-coil, DNA binding, and linker domains that mediate nuclear translocation and export, as well as Src homology 2 (SH2) and C-terminal transactivation domains that are subject to phosphorylation. Alternative splicing of STAT3 pre-mRNA leads to the formation of the full-length isoform STAT3alpha, as well as STAT3beta, a truncated isoform that lacks a portion of the transactivation domain and is considered a dominant-negative regulator of STAT3 transcriptional activation. STAT3 signaling is inhibited by the negative regulator suppressor of cytokine signaling 3 (SOCS-3) and prevents excessive STAT3 activation. Phosphorylation of STAT3 at tyrosine 705 (Tyr705) is mediated by JAKs in response to stimulation with cytokines or growth factors and induces STAT3 dimerization and nuclear translocation, leading to STAT3-dependent gene transcription. Tumor STAT3 (phospho-Tyr705) levels are increased in patients with squamous or renal cell carcinoma and are associated with metastasis. Cayman's STAT3alpha/beta (Phospho-Tyr705) Rabbit Monoclonal Antibody (Clone RM261) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.Synonyms: Signal Transducer and Activator of Transcription 3. Immunogen: Peptide corresponding to human STAT3alpha/beta (phospho-Tyr705). Clone Designation: RM261. Formulation: (Request formulation change), 100 µl of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: IHC, WB. Cross Reactivity: (+) STAT3alpha (phospho-Tyr705), (+) STAT3beta (phospho-Tyr705), (-) STAT3 without phosphorylation at Tyr705.