Anti-phospho-Vinculin (Tyr822)

Vinculin is an ubiquitously expressed cytoskeletal protein (~130kD) involved in cell adhesion and cell migration. The vinculin protein consists of a globular head domain connected to an elongated tail region by a proline-rich domain. The head region contains binding sites for two cytoskeletal proteins, a-actinin and talin, as well as a binding site for the tail region of vinculin itself. The linker region possesses binding sites for two adaptor proteins, vinexin and ponsin, that may connect growth factor receptors with integrin signaling pathways, and a binding site for an actin-bundling protein, VASP. The tail region contains binding sites for actin, the cytoskeletal protein, paxillin, and PI(4,5)P2. In the inactive state the head region of vinculin is bound to the tail region, resulting in inaccessibility of the other protein binding sites. Binding of PI(4,5)P2 releases the head-tail interaction allowing binding of other proteins to vinculin. These regulatory events play an important role in the formation, maintenance, and breakdown of focal adhesions that occur during cell adhesion and migration. In addition to these protein binding sites, the head and tail regions of vinculin have multiple potential phosphorylation sites. Data indicate the possible involvement of phosphorylation of tyrosine 822 of vinculin with the integrity/strength of integrin mediated focal adhesions. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 , Optimal dilutions to be determined by the researcher. Positive Control: Chick Embryo Fibroblasts (CEFs) transfected with activated Src, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.