Anti-phospho-Tau (Ser404) / MAPT, clone 31M20

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. Phospho-Tau (Ser404) antibody detects tau protein (MAPT) when phosphorylated at serine 404, a modification linked to neurofibrillary tangle formation. Tau is a microtubule-associated protein that stabilizes neuronal cytoskeletal structure, but abnormal phosphorylation reduces binding affinity for microtubules. Hyperphosphorylated tau aggregates into paired helical filaments, driving pathology in Alzheimer's disease and other tauopathies.Research with Phospho-Tau (Ser404) antibody has highlighted Ser404 phosphorylation as a marker of late-stage tau pathology. This site is often phosphorylated concurrently with Ser396, a combination strongly correlated with neurodegenerative disease severity. Kinases such as GSK-3beta and MAPKs regulate this modification, while phosphatase activity determines its removal. By tracking Ser404 phosphorylation, investigators can assess disease progression and evaluate therapeutic strategies aimed at modifying tau phosphorylation dynamics.In addition to Alzheimer's disease, Ser404 phosphorylation has been studied in frontotemporal dementia, progressive supranuclear palsy, and traumatic brain injury models. Its appearance in disease states provides an important readout for tau aggregation and neuronal loss. Detecting phospho-tau at this site offers insight into both pathological cascades and experimental treatments targeting tau kinases or aggregation.Antibodies specific to phospho-tau (Ser404) are validated for western blot, immunohistochemistry, and immunofluorescence. These reagents allow selective recognition of tau phosphorylated at Ser404, distinguishing it from total tau. Researchers rely on clone-based antibodies for reproducibility in studies of human tissue, animal models, and cultured neurons.NSJ Bioreagents supplies this Phospho-Tau (Ser404) antibody for investigations into tau biology, neurodegeneration, and therapeutic development.