Anti-phospho-Phospholipase C gamma 1 (Tyr1253) / PLCG1, clone 32P05

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. Phospho-Phospholipase C gamma 1 (Tyr1253) antibody targets phospholipase C gamma 1 (PLCG1) when phosphorylated at tyrosine 1253. PLCG1 is an essential enzyme in intracellular signal transduction, catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate into diacylglycerol and inositol 1,4,5-trisphosphate. These second messengers trigger activation of protein kinase C and mobilization of intracellular calcium, respectively, initiating diverse downstream cellular responses.Phosphorylation of PLCG1 at tyrosine 1253 is mediated by receptor tyrosine kinases and non-receptor tyrosine kinases. This phosphorylation event enhances catalytic activity and contributes to cell proliferation, differentiation, migration, and survival. Dysregulation of PLCG1 phosphorylation is implicated in oncogenesis, autoimmune disease, and vascular disorders.Phospho-Phospholipase C gamma 1 (Tyr1253) antibody provides precise detection of the phosphorylated form, enabling researchers to evaluate signaling activation downstream of growth factor receptors such as EGFR, PDGFR, and VEGFR. It is commonly applied in studies involving cancer biology, immune receptor signaling, and angiogenesis.This antibody is highly suitable for methods such as western blot, immunohistochemistry, immunofluorescence, and flow cytometry. By differentiating phosphorylated PLCG1 from total protein levels, it provides insight into dynamic cellular signaling events.PLCG1 phosphorylation is especially important in cancer biology, where hyperactivation of growth factor signaling drives uncontrolled proliferation. Monitoring tyrosine 1253 phosphorylation provides mechanistic understanding of oncogenic transformation and therapeutic responses to kinase inhibitors. In immunology, PLCG1 phosphorylation regulates antigen receptor signaling, impacting T cell and B cell responses.Phospho-Phospholipase C gamma 1 (Tyr1253) antibody from NSJ Bioreagents is a valuable reagent for researchers studying tyrosine kinase signaling, cell growth regulation, and immune function. Its phospho-specific recognition ensures accurate assessment of pathway activation in health and disease.