Anti-phospho-Histone H3 (Ser10), clone MSVA-903M

Antibody in 1X PBS with 0.05% BSA, 0.05% sodium azide. Histone H3 (HIST1H3A) is a nucleosomal protein that undergoes phosphorylation at serine 10 during mitosis, marking cells actively undergoing chromatin condensation. Phospho-Histone H3 (pSer10) Antibody for IHC / HIST1H3A Immunohistochemistry Antibody (clone MSVA-903M) is specifically positioned for detection of this modification in formalin-fixed, paraffin-embedded tissue sections and large-scale tissue microarray studies. This antibody is part of a broader collection of Histone H3 antibodies used to study chromatin structure, histone modifications, and epigenetic regulation.HIST1H3A antibody, also referred to as Histone H3 antibody and phospho-H3 Ser10 antibody in the literature, identifies a nuclear phosphorylation event restricted to mitotic cells. In FFPE tissues, this produces strong, sharply defined nuclear staining in mitotic figures, while surrounding non-dividing cells remain largely negative, enabling clear morphological interpretation.This Phospho-Histone H3 (pSer10) Antibody for IHC is uniquely positioned for tissue-based proliferation assessment, with a strong emphasis on histological context rather than biochemical response. In human tissue microarray (TMA) formats, staining highlights mitotically active cells across a wide range of normal and cancer tissues, allowing direct comparison of proliferation patterns across multiple tissue types within a single experiment.The nuclear staining pattern is consistent with chromatin localization, showing dense labeling of condensed chromosomes in mitotic cells. This enables straightforward identification of mitotic figures within complex tissue architecture, including epithelial, stromal, and tumor compartments. Increased staining is commonly observed in rapidly proliferating tumors, while normal tissues show more restricted labeling confined to specific proliferative zones.Unlike phosphorylation assays designed for signaling response studies, this antibody emphasizes tissue performance and interpretability in FFPE sections. The clear nuclear signal, low background, and compatibility with TMA workflows make it well suited for pathology-oriented analysis and large-scale tissue screening applications.Clone MSVA-903M antibody supports reliable detection of phospho-Histone H3 in tissue sections, enabling detailed evaluation of mitotic activity and spatial distribution of proliferating cells in both normal and diseased tissues.This antibody is also part of a broader collection of IHC antibodies validated by tissue microarray analysis, supporting consistent staining across normal and cancer tissues.