Anti-phospho-Histone H2A.X, (Ser139/Tyr142) (H2a/x, H2AFX, H2AX)

Anti-phospho-Histone H2A.X, (Ser139/Tyr142) (H2a/x, H2AFX, H2AX)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
H5110-03W.100 100 µl - -

1 - 19 business days

543.00€
 
Histone H2A.X is a variant histone that represents approximately 10% of the total H2A histone... more
Product information "Anti-phospho-Histone H2A.X, (Ser139/Tyr142) (H2a/x, H2AFX, H2AX)"
Histone H2A.X is a variant histone that represents approximately 10% of the total H2A histone proteins in normal human fibroblasts (1). H2A.X is required for checkpoint-mediated cell cycle arrest and DNA repair following double-stranded DNA breaks (1). DNA damage, caused by ionizing radiation, UV-light, or radiomimetic agents, results in rapid phosphorylation of H2A.X at Ser139 by PI3K-like kinases, including ATM, ATR and DNA-PK (2,3). Within minutes following DNA damage, H2A.X is phosphorylated on Ser139 at sites of DNA damage (4). This very early event in the DNA-damage response is required for recruitment of a multitude of DNA-damage response proteins, including MDC1, NBS1, RAD50, MRE11, 53BP1 and BRCA1 (1). In addition to its role in DNA-damage repair, H2A.X is required for DNA fragmentation during apoptosis and is phosphorylated by various kinases in response to apoptotic signals. H2A.X is phosphorylated on Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation (5-8). H2A.X is constitutively phosphorylated on Tyr142 in undamaged cells by WSTF (Williams-Beuren syndrome transcription factor) (9,10). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 1:100 , Flow Cytometry: 1:200, Western Blot: 1:1000 , Immunoprecipitation: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: Anti-phospho-Histone H2A.x
Supplier: United States Biological
Supplier-Nr: H5110-03W

Properties

Application: FC, IF, IP, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Synthetic phosphopeptide corresponding to residues surrounding the phosphorylated Ser139 and Tyr142 of human H2A.X.
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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