Anti-phospho-Histone H2A.X, gamma, (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X, H2AF

Anti-phospho-Histone H2A.X, gamma, (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X, H2AF
Item number Size Datasheet Manual SDS Delivery time Quantity Price
H5110-08V4.100 100 µl - -

3 - 19 business days*

762.00€
 
Histone H2AX is a 14kD ubiquitous member of the H2A histone family. It contains an evolutionary... more
Product information "Anti-phospho-Histone H2A.X, gamma, (Ser139) (H2A Histone Family Member X, H2A.FX, H2A/X, H2AF"
Histone H2AX is a 14kD ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated to yield a form known as gamma-H2AX in response to double-strand DNA damage. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry, Flow Cytometry and Western Blot. Not recommended for use in Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:500-1:5000, Immunohistochemistry (frozen): 1:1000-1:5000. , Immunohistochemistry (Paraffin): 1:1000-1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Formaldehyde fixation followed by permeabilization with Triton-X 100., Flow Cytometry: 5ug/1e6 cells in a 150ul reaction, Western Blot: 1:2000-1:10,000, Optimal dilutions to be determined by the researcher. Positive Control: Human HEK293 nuclear extracts, breast carcinoma, HeLa cells, ovarian carcinoma, testicular seminoma. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: Anti-phospho-H2AX, Anti-phospho-H2a/x, Anti-phospho-H2AFX, Anti-phospho-Histone H2AX, Anti-phospho-Histone H2A.X
Supplier: United States Biological
Supplier-Nr: H5110-08V4

Properties

Application: FC, IF, IHC, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Dog, Human, Mouse
Immunogen: A synthetic peptide made to a portion of the C-terminus of human histone gamma-H2AX surrounding phosphorylated serine 139.
Purity: Purified
Format: Purified

Handling & Safety

Storage: +4°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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