Anti-phospho-Erythropoietin Receptor (Tyr426) (EPOr, EPO-R)

Erythropoietin a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the erythropoietin receptor (Epo R). A member of the hematopoietic growth factor receptor superfamily which includes IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, GM-CSF, G-CSF, Thrombopoietin, LIF, CNTF, Growth Hormone, and Prolactin, Epo R is expressed not only by erythroid cells but also by embryonic stem cells, endothelial cells, and neural cells. Mouse Epo R cDNA encode a type I membrane protein with 507aa residues. Mouse Epo R has a 24aa hydrophobic signal peptide, a 225aa extracellular domain, a 22aa transmembrane domain, and a 236aa intracellular domain. At the protein sequence level, the human Epo R is ~82% identical to the mouse protein. Mouse and human Epo R both contain 11 cysteine residues and an N-linked glycosylation site. Mouse Epo R, however, contains two disulfide bridges not found with human Epo R. In common with other hematopoietic growth factor receptor superfamily members, mouse Epo R has 4 positionally conserved cysteines in its extracellular domain, a tryptophan-serine-X- tryptophan-serine (WSXWS) motif or its homolog located near the transmembrane region, and lacks kinase motifs in its intracellular domain. Based on its aa composition the molecular weight of Epo R would be 55kD but after post translational modification including glycosylation and tyrosine and serine-threonine phosphorylation the molecular weight can be as high as 78kD. As a result of alternative splicing of the Epo R mRNA, cDNA clones encoding a truncated form of the Epo R as well as a soluble form of Epo R have been found. The presence of a soluble form of the Epo R has also been detected in human serum. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.