Anti-Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody (Clone 11D3)

Histone H3 is a nuclear protein and a component of the nucleosome core that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications, including citrullination. Histone H3 is subject to citrullination of arginine residues by peptidyl arginine deiminase 4 (PAD4) at positions 2, 8, 17 and 26, and this citrullination blocks methylation of these residues by protein-arginine methyltransferase 4 (PRMT4) and induces transcriptional repression of target genes. Citrullinated histone H3 is a component of neutrophil extracellular traps (NETs) that acts as an autoantigen to induce production of anti-citrullinated protein antibodies associated with various diseases such as sepsis, multiple sclerosis, rheumatoid arthritis, and multiple myeloma. Cayman's Histone H3 (Citrullinated R2 + R8 + R17) Monoclonal Antibody can be used for ELISA and Western blot applications. The antibody recognizes citrullinated histone H3 at approximately 16 kDa from human samples.Immunogen: Histone H3 peptide with citrulline at R2, R8, and R17. Clone Designation: 11D3. MW: ~16 kDa. Formulation: (Request formulation change), 100 µg protein G-purified IgG. Storage Buffer: PBS, pH 7.2 with 50% glycerol and 0.02% sodium azide. Host: Mouse. Isotype: IgG1K. Applications: ELISA and WB. Cross Reactivity: (-) Non-citrullinated histone H3. UniProt Accession #: P68431.