Anti-Histone H2BS14Ph Monoclonal Antibody (Clone RM238)

Histone H2B is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains a histone fold domain with a C-terminal alpha-helix that facilitates nucleosome interactions and chromatin compaction, as well as an unstructured N-terminal tail that extends outside of the nucleosome core, both of which are subject to various post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation. Phosphorylation of histone H2B at serine 14 (H2BS14Ph) is associated with DNA damage and induces apoptotic chromatin condensation. H2BS14Ph also correlates with class switch recombination and somatic hypermutation in germinal center B cells. Cayman's H2BS14Ph Monoclonal Antibody (Clone RM238) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.Synonyms: H2B (Phospho-Ser14), H2BpS14, H2BpSer14, Phospho-Histone H2B Serine 14, Phosphorylated Histone H2B Serine 14. Immunogen: Peptide corresponding to H2BS14Ph. Clone Designation: RM238. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) H2BS14Ph, (-) Other phosphorylated histones.