Anti-ZNRF2

domain:The RING-type zinc finger domain is required for E3 ligase activity.,function:May play a role in the establisment and maintenance of neuronal transmission and plasticity via its ubiquitin ligase activity. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.,pathway:Protein modification, protein ubiquitination.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Present in presynaptic plasma membranes in neurons.,subunit:Interacts with UBE2N.,tissue specificity:Highly expressed in the brain, with higher expression during development than in adult. Expressed also in mammary glands, testis, colon and kidney., Protein function: E3 ubiquitin-protein ligase that plays a role in the establishment and maintenance of neuronal transmission and plasticity. Ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit/ATP1A1 and thereby influences its endocytosis and/or degradation (PubMed:22797923). Acts also as a positive regulator of mTORC1 activation by amino acids, which functions upstream of the V-ATPase and of Rag-GTPases (PubMed:27244671). In turn, phosphorylation by mTOR leads to its inhibition via targeting to the cytosol allowing a self-regulating feedback mechanism (PubMed:27244671). [The UniProt Consortium] Recommended dilutions: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.. Cellular localization: Endosome membrane , Peripheral membrane protein . Lysosome membrane , Peripheral membrane protein . Cell junction, synapse, presynaptic cell membrane , Peripheral membrane protein .