Anti-Zinc Transporter ZIP2 (Solute Carrier Family 39 Member 2, Zrt- and Irt-like Protein 2, ZIP-2, h

Zinc is an essential nutrient for all organisms because of the many important roles this metal plays. Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins. In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters. In mammals, the Zip1, Zip2, Zip3, Zip4, Zip5, LIV- 1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types. ZIP2 (SLC39A2, Eti-1, 6A1) encodes a protein of 309aa, includes an amino terminal signal peptide of 26aa, and has the predicted topology of Zip proteins. The human or mouse Zip2 overexpression in transfected K562 and human embryonic kidney (HEK293) cells, respectively, increased 65Zn uptake in these cells. hZIP2-induced Zn uptake was shown to be highly inhibited by Mn2+ and Co2+, metals that did not substantially affect hZIP1-, mZIP1-, or mZIP2-induced Zn uptake. Zn uptake mediated by hZIP2 appears to be stimulated by HCO?3, non-ATP dependent, but sensitive to N-ethylmaleimide, a sulfhydryl-reactive agent. , Applications:, Suitable for use in Western Blot, ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml (Chemiluminescence), ELISA: 1:100,000, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.