Anti-Zebrafish Yb-1 / Ybx1 / Y-box-binding protein 1

0.5mg/ml if reconstituted with 0.2ml sterile DI water. Y-box binding protein 1 (Ybx1, Yb-1) is a multifunctional RNA- and DNA-binding protein conserved across vertebrates. In zebrafish, Yb-1 plays a critical role in early embryogenesis, cellular proliferation, and gene regulation. The protein contains a conserved cold shock domain (CSD) that facilitates binding to nucleic acids, enabling Yb-1 to modulate transcription, mRNA splicing, stability, and translation.Biological Function: Yb-1 is essential for zebrafish embryonic development. Knockdown or mutation of Yb-1 results in early embryonic lethality due to impaired morphogenesis, suggesting a key regulatory role in zygotic gene activation and maternal RNA clearance. It is also involved in stress responses, regulation of cell cycle genes, and may contribute to sex determination pathways in zebrafish.Expression Pattern:Yb-1 is maternally deposited and ubiquitously expressed during early embryonic stages. As development progresses, its expression becomes more tissue-specific, particularly enriched in proliferative zones and neural tissues.Orthology and Conservation:Zebrafish Ybx1 is orthologous to human YBX1, sharing high sequence similarity and functional conservation. This makes zebrafish a valuable model for studying YBX1-related roles in development and disease, including cancer biology and RNA regulation. Protein function: DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing and transcription regulation (PubMed:30135188, PubMed:31399345). Binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C) (PubMed:31399345). Promotes mRNA stabilization: acts by binding to m5C- containing mRNAs and recruiting pabpc1a, thereby preventing mRNA decay (PubMed:31399345). Plays a role in the maternal-to-zygotic transition in early embryo by binding to m5C-containing maternal mRNAs and preventing their degradation (PubMed:31399345). Also promotes maternal- to-zygotic transition in oocytes and embryos by promoting translation repression, molecular mechanisms governing translation repression are unknown (PubMed:30135188). Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs. Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C)- containing RNAs. Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence: acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs. Also involved in pre-mRNA alternative splicing regulation: binds to splice sites in pre-mRNA and regulates splice site selection. Also able to bind DNA and regulate transcription. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'). Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA, suggesting a role in DNA repair. The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. [The UniProt Consortium]