Anti-Zebrafish Mcad / Acadm

0.5mg/ml if reconstituted with 0.2ml sterile DI water. Medium-chain acyl-CoA dehydrogenase (MCAD) is a mitochondrial enzyme that plays a central role in the beta-oxidation of medium-chain fatty acids. Encoded by the acadm gene, MCAD catalyzes the initial dehydrogenation step in the metabolism of fatty acids with chain lengths of 6 to 12 carbons, producing enoyl-CoA derivatives and transferring electrons to the electron transport chain via electron transfer flavoprotein (ETF).In zebrafish (Danio rerio), MCAD is highly conserved and functionally analogous to its mammalian counterparts, making it an ideal model for studying fatty acid metabolism and mitochondrial energy homeostasis in vertebrates. Zebrafish mcad is expressed during early development and in metabolically active tissues such as muscle, liver, and heart. Disruption of mcad function in zebrafish has been used to model human MCAD deficiency, a common inherited metabolic disorder that can lead to hypoglycemia, lethargy, and sudden infant death.Due to its evolutionary conservation and physiological relevance, zebrafish MCAD is a valuable tool for research into metabolic diseases, mitochondrial function, and energy regulation in vivo. Protein function: Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF- ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains. [The UniProt Consortium]