Anti-XC Chemokine Receptor 1 (G Protein Coupled Receptor 5, Lymphotactin Receptor, SCM-1, Single Cys

XC Chemokine Receptor 1 is a 38kD member of the G-protein coupled receptor 1 family. It binds XCL1/lymphotactin/SCM-1 alpha and XCL2/SCM-1 beta. In addition, human herpes virus 8 (HHV8) encodes two viral chemokines vCCL2/vMIP-II and vCCL3/vMIP-III that function as an antagonist and a highly selective agonist, respectively, for XC Chemokine Receptor 1. XC Chemokine Receptor 1 is expressed on neutrophils, CD8+ T cells , NK cells, CD4+ T cells and B cells. Human XC Chemokine Receptor 1 is a 333aa, 7-transmembrane molecule. It contains a 32aa N-terminus that lacks glycosylation sites and a 42aa C-terminal cytoplasmic tail. Over the extracellular regions used for immunization, human XC Chemokine Receptor 1 shares 62%, 54% and 64% aa identity with canine, mouse and porcine XCR1, respectively. Applications: Suitable for use in Immunocytochemistry, Flow Cytometry, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 15ug/ml , Flow Cytometry: 25ug/ml, 10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul. Tested using LPS-treated PBMCs., Western Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions., Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.5ng/well., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.