Anti-WDR4 / WD repeat domain 4, clone 25W87

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. WDR4 antibody detects WD repeat domain 4, a conserved protein involved in RNA modification and tRNA methylation. WDR4 forms a complex with METTL1 to catalyze the N7-methylguanosine modification of tRNAs, a modification required for translational fidelity and RNA stability. The WDR4 antibody is widely used in molecular biology and RNA research to study RNA methylation, protein translation, and gene expression regulation.WDR4 is encoded by the WDR4 gene located on human chromosome 21q22.3. The protein is approximately 412 amino acids long and contains several WD40 repeats that mediate protein-protein interactions, forming a beta-propeller structure typical of the WD repeat family. WDR4 localizes to the nucleus and cytoplasm, where it partners with METTL1 to catalyze methylation of specific tRNA guanine residues, ensuring proper translation initiation and elongation.The WDR4 antibody detects a 46 kilodalton protein by western blot and demonstrates nuclear and cytoplasmic staining patterns under immunofluorescence. Through its interaction with METTL1, WDR4 stabilizes the tRNA methyltransferase complex and regulates global protein synthesis. Loss of WDR4 leads to reduced tRNA methylation, impaired translation, and increased sensitivity to stress.Mutations in WDR4 cause a spectrum of developmental and neurological disorders, including microcephaly and intellectual disability. These defects arise from disrupted tRNA modification, which affects the translation of growth-related and neuronal genes. In cancer, overexpression of WDR4 enhances translation of oncogenic proteins and promotes tumor progression through metabolic reprogramming.Because of its key role in RNA modification and translation regulation, WDR4 serves as an essential model for understanding epitranscriptomic control of gene expression. NSJ Bioreagents provides a validated WDR4 antibody optimized for its applications, supporting studies of RNA methylation, translational regulation, and disease pathogenesis.